0 ratings
Vare #:
145284746
NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL
Vare #:
145284746
DKK 492
Prisdetaljer
Eksklusive forsendelses- og toldgebyrer ( Forsendelses- og toldgebyrer beregnes ved checkout )
*Alle varer importeres fra US
0 ratings
Skriv en anmeldelse
På lager
Importeret fra USA butik
Bestil nu og få den omkring Torsdag, September 03
Sikker transaktion
Ubuy arbejder hårdt for at beskytte din sikkerhed og dit privatliv. Vores avancerede betalingssikkerhedssystem sikrer fortrolighed ved at kryptere dine oplysninger under transmission ved hjælp af AES (Advanced Encryption Standards) og SSL (Secure Socket Layer) protokoller. Dine betalingsoplysninger er 100% sikre, da vi ikke deler dine betalingsoplysninger med tredjepartssælgere.
Vores bedste logistikpartnere
Manufactured in an ISO 9001 and 13485 certified facility in the USA, ensuring the highest quality for your research needs.
Hurtig
levering
Gratis
returnering*
Sikker emballering
100 % originale produkter'
PCI DSS Compliance
ISO 27001 Certified
Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Køb nu.
Hvad der skiller sig ud
Dual Concentration
The NP40 Cell Lysis Buffer is formulated at a 2X concentration, ensuring efficient and effective cell lysis in fewer steps, saving time for researchers while maintaining optimal results.
pH Stability
Maintaining a pH of 7.4, this buffer provides a controlled environment, crucial for preserving protein integrity during lysis, making it ideal for sensitive experiments and applications.
Generous Volume
With 250mL of solution, there is ample buffer for numerous applications, reducing the frequency of repurchasing and ensuring that labs can perform multiple experiments seamlessly.
Produktdetaljer
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 250 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.112 |
| Brand | eCon Lab Supply |
| Item Volume | 250 ml |
| Package Information | Bottle |
Hvem bør købe?
-
Molecular Biologists
Ideal for extracting proteins from cells to facilitate downstream molecular biology applications.
-
Biochemistry Laboratories
Useful in enzymatic studies where cell lysis is needed to release cellular components.
-
Research Institutions
Beneficial for researchers conducting studies on cellular processes and protein interactions.
-
General Consumers
Not suitable for non-laboratory individuals due to specialized usage and safety considerations.
Produktbeskrivelse
NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL
Har du spørgsmål? Chat med os
Kundespørgsmål og svar
-
spørgsmål:
What is NP40 Cell Lysis Buffer and how is it used?
svar: NP40 Cell Lysis Buffer is a specialized solution designed for the efficient lysis of cells to extract cellular components such as proteins and nucleic acids. It operates at a neutral pH of 7.4 and is recommended for use in cell biology experiments where high yield and purity are critical. This buffer can disrupt both cell membranes and nuclear membranes, effectively releasing contents for further analysis. It is widely used in research laboratories for applications like Western blotting, immunoprecipitation, and enzyme assays. -
spørgsmål:
What are the key components of NP40 Cell Lysis Buffer?
svar: The NP40 Cell Lysis Buffer contains Nonidet P-40 (NP-40), a non-ionic detergent, along with salts and buffering agents that maintain the pH at 7.4. These components work synergistically to solubilize membrane proteins without denaturing them, which is crucial for downstream applications like SDS-PAGE or mass spectrometry. The balance of these ingredients is tailored to provide optimal lysis conditions while preserving protein functionality, making it a popular choice for life science research. -
spørgsmål:
Can NP40 Cell Lysis Buffer be used for different cell types?
svar: Yes, NP40 Cell Lysis Buffer is versatile and can be applied to various cell types, including mammalian, yeast, and bacterial cells. Its formulation allows for efficient lysis regardless of the cellular composition. This makes it an excellent tool in diverse experimental setups, such as studying protein interactions in mammalian cells or extracting plasmids from bacterial cells. However, it's recommended to optimize conditions based on specific cell types and experimental goals. -
spørgsmål:
How should NP40 Cell Lysis Buffer be stored?
svar: NP40 Cell Lysis Buffer should be stored at 4°C to maintain its stability and effectiveness. For longer storage, aliquoting the buffer into smaller tubes can help minimize repeated freeze-thaw cycles, which may compromise its integrity. Always check for any signs of precipitation or discoloration before use. Proper storage extends its viability, ensuring consistent performance in experiments over time, especially in high-throughput applications. -
spørgsmål:
What precautions should be taken while using NP40 Cell Lysis Buffer?
svar: When using NP40 Cell Lysis Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye contact with the buffer. Additionally, work in a well-ventilated area to avoid inhaling vapors. Ensure that all reagents are at the correct temperature prior to use and that your lab solutions are prepared under sterile conditions to prevent contamination. These precautions ensure a safe and controlled experimental environment. -
spørgsmål:
How does NP40 Cell Lysis Buffer affect protein assays?
svar: NP40 Cell Lysis Buffer is designed to maintain protein stability while extracting them from cells. It minimizes protein denaturation, which is crucial for assays such as Western blotting or ELISA. This buffer allows for the recovery of functional proteins, making it highly suitable for assays where protein activity is determined. Users often find that using this buffer leads to more consistent and reproducible results in their analyses. -
spørgsmål:
Is NP40 Cell Lysis Buffer suitable for DNA extraction?
svar: While NP40 Cell Lysis Buffer is primarily developed for protein extraction, it can also aid in DNA extraction by breaking down cell membranes that house both proteins and nucleic acids. However, to ensure purity, it's advisable to perform a phenol-chloroform extraction following lysis. This approach allows for the recovery of high-quality DNA suitable for applications such as PCR. Researchers often use this buffer to facilitate simultaneous protein and DNA study within the same sample. -
spørgsmål:
Are there any alternatives to NP40 Cell Lysis Buffer?
svar: Yes, there are several alternatives to NP40 Cell Lysis Buffer, including Triton X-100 and SDS-based lysis buffers. Each of these alternatives may have specific advantages depending on the application. For instance, Triton X-100 is milder and less likely to disrupt protein structure, making it suitable for applications requiring intact proteins. In contrast, SDS is more effective for complete denaturation of proteins. Researchers often choose a buffer based on their specific experimental needs and the type of analysis they plan to perform. -
spørgsmål:
How much NP40 Cell Lysis Buffer should I use per sample?
svar: The optimal volume of NP40 Cell Lysis Buffer typically depends on the type and number of cells being lysed. A general recommendation is to use 100-300 µL of the buffer per 1-10 million cells. Adjusting the volume may be necessary based on cell density and the specific requirements of your assay. Researchers find that using the right amount ensures efficient lysis while preventing the dilution of key cellular components, leading to more reliable experimental outcomes. -
spørgsmål:
Where can I buy NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL in Faroe Islands?
svar: You can purchase NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL from Ubuy in Faroe Islands. Ubuy offers a reliable platform for sourcing laboratory reagents and supplies, making it a convenient choice for researchers and scientists needing high-quality buffers and reagents. Ensure you check their website for availability and details on your specific needs.
eCon Lab Supply Buffers Editorial Review
Ingen redaktionelle anmeldelser fundet
Kundeanmeldelser & vurderinger
1 kundebedømmelser
-
5 stjerne
0%
-
4 stjerne
100%
-
3 stjerne
0%
-
2 stjerne
0%
-
1 stjerne
0%
Anmeld dette produkt
Del dine tanker med andre kunder
Platformstillid & Køberens Tillid
MA
Mohammad
Verificeret køber
“Excellent quality and original too,when ubuy send original things I will appreciate that ,I very satisfied thank you”
23 July 2026 · via Trustpilot
CK
Chitra
Verificeret køber
“The order and delivery progress was communicated very well. Package arrived within the estimated time. Products arrived as expected in good condition.”
22-Jul-26 · via Trustpilot
K
kund
Verificeret køber
“Good supply of products. Safe payment methods, and shipment worldwide! Genuine products.”
23-Jul-26 · via Trustpilot
OO
Onesi
Verificeret køber
“Was my first time buying a product from Ubuy, but I found it so helpful. This is reliable. Gonna place a new order!”
22 July 2026 · via Trustpilot
SJ
SAO JOHN
Verificeret køber
“Ubuy is a great online platform to buy stuff. Reliable, fast delivery time and great value for money. I've been using Ubuy online shopping platform for three years now and will continue to do so.”
23 July 2026 · via Trustpilot
Sikker betaling
Global levering
Nem returnering
Originale produkter
Produktets prishistorik
Vigtig information
- Begrænsninger: For produkter, der forsendes internationalt, bemærk venligst, at eventuel producentgaranti muligvis ikke er gyldig; producentens serviceordninger er muligvis ikke tilgængelige; produktmanualer, instruktioner og sikkerhedsadvarsler er muligvis ikke på destinationslandets sprog; produkterne (og det medfølgende materiale) er muligvis ikke designet i overensstemmelse med destinationslandets standarder, specifikationer og mærkningskrav; og produkterne opfylder muligvis ikke destinationslandets krav til spænding og andre elektriske standarder (hvilket kan kræve brug af en adapter eller transformer, hvis relevant). Modtageren er ansvarlig for at sikre, at produktet lovligt kan importeres til destinationslandet. Ved bestilling hos Ubuy eller dets datterselskaber er modtageren importøren og skal overholde alle love og regler i destinationslandet.
- Det er ikke alle de produkter som vises på Ubuy som er til salg, idet Ubuy er en global søgemaskine. Produkterne er underlagt eksport-/handelsregler.
DKK 492
Bestil nu og få den omkring Torsdag, September 03
Dette produkt er ikke begrænset i mit land. (Klik venligst på linket ovenfor, hvis dette produkt ikke er begrænset i dit land, så vil vores team gennemgå det og godkende det.)
QTY:
Sikker transaktion
PCI DSS-kompatibel og ISO 27001:2022-certificeret, med krypteret betaling og fuld køberbeskyttelse på hver ordre
Vores bedste logistikpartnere
Hurtigste grænseoverskridende levering
Egenskaber og fordele
- Comprehensive range of biological buffers.
- Ideal for research, diagnostics, drug development, and manufacturing.
- Certified facility guarantees top-tier quality.
- Designed for protein research and molecular biology.
- NP40 Cell Lysis Buffer is suitable for key assays like ELISA and Western blotting.
- Strict adherence to Good Manufacturing Practices ensures reliability.
Ubuy-garanti
Oplev bekymringsfri shopping med 100 % originale produkter, PCI DSS-kompatibel betalingssikkerhed, ISO 27001-certificeret databeskyttelse, den hurtigste grænseoverskridende levering, gratis returnering* og sikker emballage på hver ordre.