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Vare #:
85519151
50X TAE Buffer (Tris-Acetate-EDTA), 100 mL
Vare #:
85519151
DKK 336
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Importeret fra USA butik
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Vores bedste logistikpartnere
50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.
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Produktdetaljer
| Item Weight | 0.22 lbs (100 grams) |
PRODUKTBESKRIVELSE
50X TAE Buffer (Tris-Acetate-EDTA), 100 mL
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Kundespørgsmal & svar
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spørgsmål:
What is 50X TAE Buffer Tris-Acetate-EDTA used for?
svar: 50X TAE Buffer Tris-Acetate-EDTA is primarily used in molecular biology for nucleic acid electrophoresis. This concentrated buffer allows for efficient separation of DNA and RNA in gel electrophoresis techniques. When diluted, it maintains the pH and ionic environment necessary for accurate results during DNA analysis, cloning, and other genetic applications. -
spørgsmål:
How do you prepare 1X TAE Buffer from 50X concentration?
svar: To prepare 1X TAE Buffer from 50X concentration, dilute the buffer by mixing 1 part of 50X TAE with 49 parts of distilled water. This results in a final volume where the buffer is at the appropriate dilution for running gel electrophoresis, ensuring the components are balanced for optimal performance in your experiments. -
spørgsmål:
Why is Tris-Acetate-EDTA preferred over other buffers?
svar: Tris-Acetate-EDTA (TAE) is favored for its ability to provide consistent buffering capacity and low electroendosmosis during electrophoresis. Unlike other buffers, TAE allows for the recovery of nucleic acids post-electrophoresis as it does not interfere with downstream applications, making it ideal for both analysis and cloning of DNA. -
spørgsmål:
Can TAE Buffer be reused after gel electrophoresis?
svar: Generally, TAE Buffer may be reused if it remains clean and free from contaminants. However, repeated use can compromise the buffer's pH and ionic strength, affecting the results of subsequent experiments. Always evaluate the quality of the buffer after each use and consider preparing fresh buffer for critical applications to maintain accuracy. -
spørgsmål:
What are the storage conditions for 50X TAE Buffer?
svar: 50X TAE Buffer should be stored in a cool, dark place, ideally at room temperature or in a refrigerator to maintain its efficacy. Properly sealing the bottle after use will prevent contamination. If stored correctly, it can remain stable for an extended period, ensuring it's ready for your experimental needs. -
spørgsmål:
Is 50X TAE Buffer compatible with all types of gel electrophoresis?
svar: Yes, 50X TAE Buffer is compatible with various gel electrophoresis methods, including agarose and polyacrylamide gels. While TAE is typically used for DNA applications, it's important to adapt your experiments based on the specific type of nucleic acids being analyzed to achieve the best results. -
spørgsmål:
How does the concentration of TAE Buffer impact electrophoresis results?
svar: The concentration of TAE Buffer used directly impacts the resolution and migration speed of nucleic acids during electrophoresis. Higher concentrations can lead to slower migration and better resolution in separating fragments, while lower concentrations may generate faster migration, making it necessary to optimize based on the experiment's needs. -
spørgsmål:
What pH does 50X TAE Buffer maintain?
svar: 50X TAE Buffer typically maintains a pH around 8.0, which is optimal for most enzymatic reactions and electrophoresis processes. This pH ensures stability and functionality, providing a conducive environment for nucleic acid interactions and separation during experimental procedures in molecular biology. -
spørgsmål:
Can I use 50X TAE Buffer for RNA electrophoresis?
svar: Yes, 50X TAE Buffer can be used for RNA electrophoresis, although many prefer using 1X TBE for RNA as it offers sharper bands. However, TAE still effectively provides a stable environment for RNA separation, making it a suitable choice in many laboratory applications involving RNA. -
spørgsmål:
Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 100 mL in Faroe Islands?
svar: You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 100 mL from Ubuy, which offers a wide selection of laboratory supplies that can be delivered to your location in Faroe Islands. Ubuy ensures that you receive a product that meets your experimental requirements.
eConLabSupply Buffers Editorial Review
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Fordele
- High-quality buffer solution
- Stable and reliable performance
- Easy to use in experiments
- Excellent for electrophoresis
- Great customer reviews
Ulemper
- Some may find the size too small.
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- Begrænsninger : ved produkter som forsendes internationalt skal du venligst være opmærksom på, at eventuelle garantier fra producenten ikke nødvendigvis gælder; producentens tjenester vil måske ikke være tilgængelige; produktmanualer, instruktioner og sikkerhedsoplysninger vil måske ikke findes på destinationslandets sprog. Produkterne samt tilhørende materialer vil måske ikke være designet i overensstemmelse med destinationslandets nationale standarder, specifikationer og krav til mærkning; og produkterne lever ikke nødvendigvis op til destinationslandets standarder for spænding og strøm (hvilket betyder, at en adapter eller omformer kan være nødvendig). Køber er ansvarlig for at sikre, at produktet kan importeres på lovlig vis til destinationslandet. Ved køb hos Ubuy eller Ubuys filialer er køberen officiel importøt og skal følge alle destinationslandets love og regler.
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DKK 336
Bestil nu og få den omkring Torsdag, Juli 09
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Sikker transaktion
Ubuy arbejder hårdt for at beskytte din sikkerhed og dit privatliv. Vores avancerede betalingssikkerhedssystem sikrer fortrolighed ved at kryptere dine oplysninger under transmission ved hjælp af AES (Advanced Encryption Standards) og SSL (Secure Socket Layer) protokoller. Dine betalingsoplysninger er 100% sikre, da vi ikke deler dine betalingsoplysninger med tredjepartssælgere.
Vores bedste logistikpartnere
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Egenskaber og fordele
- Ideal for running electrophoresis of nucleic acids
- Provides electrical conductivity and maintains pH
- Protects DNA from nucleases
- Suitable for both genomic and large supercoiled DNA
- Can be used as a gel preparation buffer